Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model

Thea Fleischmann; Flora Nicholls; Miriam Lipiski; Margarete Arras; Nikola Cesarovic

doi:10.1007/978-1-4939-9473-1_20

Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model

Methods Mol Biol. 2019:1993:251-259. doi: 10.1007/978-1-4939-9473-1_20.

Authors

Thea Fleischmann^{1

2}, Flora Nicholls³, Miriam Lipiski^{4

3}, Margarete Arras⁴, Nikola Cesarovic⁴

Affiliations

¹ Division of Surgical Research, University Hospital Zurich, University of Zurich, Zurich, Switzerland. thea.fleischmann@usz.ch.
² Biological Central Laboratory, University of Zurich, Zurich, Switzerland. thea.fleischmann@usz.ch.
³ Biological Central Laboratory, University of Zurich, Zurich, Switzerland.
⁴ Division of Surgical Research, University Hospital Zurich, University of Zurich, Zurich, Switzerland.

PMID: 31148093
DOI: 10.1007/978-1-4939-9473-1_20

Abstract

Due to its similarity of skin anatomy and physiology, the pig appears to be a well-suited animal model for preclinical studies of skin analog transplantations. The choice of the location of the skin defect and appropriate postoperative measures are essential for the protection of the transplanted graft. This protocol describes in detail a porcine skin transplantation model including peri- and postoperative measures taken to improve and refine the study outcome.

Keywords: Animal model; Dermo-epidermal skin substitute; Perioperative refinement; Pig; Skin transplantation.

MeSH terms

Animals
Dermis
Epidermis
Models, Animal*
Regenerative Medicine / methods*
Skin Transplantation / methods*
Skin, Artificial*
Sus scrofa / surgery
Tissue Engineering
Transplantation, Autologous / methods*