Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to -Omics

Methods Mol Biol. 2019:1992:367-376. doi: 10.1007/978-1-4939-9469-4_25.

Abstract

Here we provide an overview of procedures for long-term cultivation, phenotyping, genotyping, and genetic transformation of cell cultures of tobacco cell lines BY-2 and VBI-0, and of A. thaliana, ecotype Landsberg erecta (LE) cell line. Notably, we present an improved protocol for BY-2 transformation and cloning and extend the available plant cell lines methodology toward high-throughput technologies like fluorescent-based cell sorting and transcriptomics.

Keywords: BY-2; Cell lines; Cell sorting; Cloning; Tobacco; Transcriptomics; Transformation; VBI-0.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / cytology*
  • Arabidopsis / genetics*
  • Cell Culture Techniques / methods
  • Cell Line
  • Cloning, Molecular / methods
  • Flow Cytometry / methods
  • Gene Expression Profiling / methods
  • Genotyping Techniques / methods
  • Nicotiana / cytology*
  • Nicotiana / genetics*
  • Plants, Genetically Modified / cytology
  • Plants, Genetically Modified / genetics
  • Transcriptome
  • Transformation, Genetic