Fluorescence Lifetime Imaging of Plant Cell Walls

Christine Terryn; Gabriel Paës

doi:10.1007/978-1-4939-9469-4_5

Fluorescence Lifetime Imaging of Plant Cell Walls

Methods Mol Biol. 2019:1992:77-82. doi: 10.1007/978-1-4939-9469-4_5.

Authors

Christine Terryn¹, Gabriel Paës²

Affiliations

¹ PICT Platform, University of Reims Champagne-Ardenne, Reims, France.
² Fractionation of AgroResources and Environment (FARE) Laboratory, INRA, University of Reims Champagne-Ardenne, Reims, France. gabriel.paes@inra.fr.

PMID: 31148032
DOI: 10.1007/978-1-4939-9469-4_5

Abstract

Fluorescence is a versatile property of many molecules called fluorophores. In plant cell walls, fluorescence is generally attributed to aromatic molecules such as lignin. In contrary to fluorescence intensity, fluorescence lifetime is independent from fluorophore concentration. So mapping fluorescence lifetime of plant cell walls represents a complementary approach to acquire chemical and structural information of cell wall components and interactions.

Keywords: Fluorescence; Lifetime; Lignin; Lignocellulose; Microscopy; Plant.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Wall / chemistry
Cell Wall / ultrastructure*
Lignin / analysis
Microscopy, Fluorescence / methods*
Optical Imaging / methods*
Plants / chemistry
Plants / ultrastructure*

Substances

lignocellulose
Lignin