In this chapter, conventional techniques are described for the preparation of plant samples at room temperature before examination in the high resolution scanning electron microscopy. Protocols are given on how to collect, to fix, to dehydrate, and to dry plant samples. Subsequently, it is described how to stick them to stubs and cover with a thin conductive layer. These methods are suitable for a wide variety of plant specimens, ranging from microalgae to higher plants.
Keywords: Chemical fixation; Critical point drying; Dehydration; High-resolution scanning electron microscope; Metal coating; Plant sample; Sample storage; t-Butanol freeze drying.