Sinusoidal voltage electrodeposition of PEDOT-Prussian blue nanoparticles composite and its application to amperometric sensing of H2O2 in human blood

Abstract

A selective electrochemical sensor based on poly(3,4-ethylenedioxythiophene) (PEDOT) - Prussian blue nanoparticles (PBNPs) for hydrogen peroxide (H₂O₂) determination was prepared by innovative sinusoidal voltage (SV) method. The successful incorporation of citrate-stabilized PBNPs into PEDOT matrix was confirmed by energy dispersive X-ray analysis (EDX), Raman spectroscopy, UV-Vis spectroelectrochemistry and cyclic voltammetry measurements. The SV preparation method provides a PEDOT-PBNPs coating with rough surface morphology and good electrocatalytic activity toward H₂O₂ reduction. The amperometric response of PEDOT-PBNPs-based sensor at -50 mV vs. Ag/AgCl is linear within the range of concentrations from 5 μM to 1 mM H₂O₂ with a detection limit of 1.4 μM H₂O₂. The proposed Pt/PEDOT-PBNPs sensor displays good repeatability, reproducibility, operational stability as well as good selectivity toward H₂O₂ determination in the presence of interfering species like dopamine (DA), uric acid (UA), KNO₂ glucose (Glu), KNO₃ and ascorbic acid (AA), and was successfully applied to H₂O₂ determination in human blood samples without biofouling.

Keywords: Composite material; Hydrogen peroxide, human blood; PEDOT; Prussian blue nanoparticles; Sinusoidal voltage.