A nanostructured catalyst is introduced that demonstrates peroxidase mimicking activity. It consists of nickel- and nitrogen-doped graphene nanotubes loaded with platinum nanoparticles. Pt-decorated Ni-doped nitrogen-rich graphitic nanotube (Pt/Ni@NGT) was synthesized using a two-step procedure in which the precursors were first refluxed to form a supramolecular assembly followed by a pyrolysis and leaching step to form nanotubes. Afterwards, Pt was decorated on the outer surface of nanotube by an ultrasound assisted method. Pt/Ni@NGT was characterized by XPS, TEM, SEM, and HAADF-STEM. The as-prepared Pt/Ni@NGT nanostructure was used for the detection of glucose via catalyzing the oxidation of a substrate, 3,3',5,5'-tetramethylbenzidine (TMB), to form a blue product (ox-TMB), thereby enabling colorimetric assay for enzymatically generated H2O2. The nanostructure exhibited excellent biocompatibility and led to highly efficient immobilization and retention of GOx. The method has a linear response in the 43 pM to 220 μM glucose concentration range, a detection limit as low as 1 pM and a limit of quantification of 3.4pM, along with good reproducibility(< 3%). A paper based visual microfluidic assay was also worked out that has an analytical range that extends from 0.1-50 mM. It is simple and rapid enough to be useful as a glucose home test.. The method was successfully applied to the determination of glucose in tear and saliva samples. Graphical abstract Graphene nanotubes doped with nitrogen and nickel (Ni@NGT) have been synthesized as the support to construct the unique Pt/Ni@NGT for providing artificial peroxidase activity for the GOx-based detection of glucose, which was further used for the construction of a glucose paper assay.
Keywords: GOx; Glucose; Nanotube; Peroxidase-like mimetic; Saliva; TMB; Tear.