Oxidative stress and endoreduplication induced by blue light exposure to CHO cells

Abstract

Blue light is commonly used for the treatment of Neonatal Jaundice and as a photodynamic therapy for cancer. In comparison to ultraviolet light, blue light has a lower toxicity due to the differences in photon energies. However, blue light can still be mutagenic to cells. The proposed mechanism suggests blue light exposure induces reactive oxygen species inducing oxidative stress. In this study, we examined how blue light exposure caused genotoxic effects utilizing Chinese hamster ovary (CHO) cells and UV135 cells when exposed to fluorescent blue light. Cytotoxic effects of blue light exposure were quantified through cellular oxidative stress analysis, cell survival assay, and in cell cycle arrest experiments. Genotoxicity was studied in sister chromatid exchange (SCE) only, and endoreduplication formation. Following blue light exposure, an increase of cell cycle arrest, oxidative stress, and cytotoxicity was observed. Blue light treatment also produced an increased amount of SCE, and more importantly, induced endoreduplicated chromosomes. In conclusion, exposure to blue light resulted in significant genotoxicity of the treated cells.

Keywords: Blue light; Cytotoxicity; Endoreduplication; Oxidative stress; Sister chromatid exchange.