Potential roles of melatonin and sulfur in alleviation of lanthanum toxicity in tomato seedlings

Abstract

Owing to the active use of rare-earth elements in many areas, it is necessary to study their behavior in the environment and their biological impact on plants. Despite the role of melatonin and sulfur in plant growth, development and abiotic stress tolerance; it is still not clear how they have a strong regulatory influence and synergistic effect on growth, physiological and biochemical characteristics of plants under different environmental stresses. Therefore, this study highlights how melatonin and sulfur together potentially involved in a reversal of lanthanum-inhibited photosynthetic and growth responses in tomato seedlings. Here, we reported that seedlings grown in a medium containing 150 μM lanthanum exhibited increased overproduction of reactive oxygen species (ROS) and lipid peroxidation together with increased Chlorophyll degradation, and activity of chlorophyllase, proline dehydrogenase and glycolate oxidase (GOx), and decreased photosynthesis and growth. However, the application of melatonin and sulfur showed significant responses on tomato seedlings, although the response of their combined treatment was more effective by further increasing photosynthesis and growth under lanthanum toxicity. Melatonin supplied with sulfur suppressed ROS formation, lipid peroxidation and activity of GOx, and increased photosynthesis by upregulating activities of carbonic anhydrase and ribulose-1,5-bisphosphate carboxylase/oxygenase. Also, sulfur supplementation with melatonin to seedlings resulted in an elevation in the accumulation of Chl and proline by increasing δ-aminolevulinic acid and activity of δ-aminolevulinic acid dehydratase and Δ¹-pyrroline-5-carboxylate synthetase activity. The administration of melatonin with sulfur substantially induced upregulation of enzymes (superoxide dismutase, ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase and glutathione reductase) activities involved in the antioxidant system, thereby mitigating ROS-induced oxidative damage. Thus, this study provides strong evidence that melatonin and sulfur have strong regulatory influence and synergistic role in alleviating the adverse effect of lanthanum-toxicity by increasing photosynthesis and growth.

Keywords: Antioxidant system; Chlorophyll degradation; Lanthanum; Melatonin; Photosynthesis; Proline biosynthesis.