Abstract
Treatment of rat granulosa cells with LHRH or the phorbol ester TPA stimulated progesterone (P) production during a 5-h incubation. The concomitant presence of a potent inhibitor of protein kinase C attenuated the LHRH effect by ca. 60% and completely blocked the stimulatory effect of TPA. Addition of TPA (10(-9), 10(-8) or 10(-7) M) or dioctanylglycerol (100 micrograms/ml) increased P production; these stimulatory effects were not potentiated by the concomitant presence of a calcium ionophore (A23187, 10(-7) or 10(-6)M). These data support the hypothesis that protein kinase C activation have a role in the steroidogenic action of LHRH in the ovary.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Animals
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Calcimycin / pharmacology
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Female
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Gonadotropin-Releasing Hormone / pharmacology*
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Granulosa Cells / drug effects
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Granulosa Cells / metabolism*
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In Vitro Techniques
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Isoquinolines / pharmacology
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Kinetics
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Phorbol Esters / pharmacology
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Piperazines / pharmacology
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Progesterone / biosynthesis*
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Protein Kinase C / antagonists & inhibitors
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Protein Kinase C / metabolism*
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Rats
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Rats, Inbred Strains
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Tetradecanoylphorbol Acetate / pharmacology
Substances
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Isoquinolines
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Phorbol Esters
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Piperazines
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Gonadotropin-Releasing Hormone
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Calcimycin
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Progesterone
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1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
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Protein Kinase C
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Tetradecanoylphorbol Acetate