iTRAQ-based proteomic analysis to identify molecular mechanisms of the selenium deficiency response in the Przewalski's gazelle

Abstract

The Przewalski's gazelle shows long-term survival in a selenium (Se)-deficient environment, but fails to exhibit obvious pathological manifestations. To reveal proteomic changes in the Przewalski's gazelle in response to Se-deficiency, twenty Przewalski's gazelle were randomly divided into control group and Se-deficient group. After induction of Se-deficiency animal model, blood samples were collected from eight animals. An isobaric tag for relative and absolute quantitation (iTRAQ)-liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomics approach was employed to explore blood protein alterations and potential mechanisms of the response to Se-deficiency challenge. Se deficiency contributed to a remarkable change in blood Se levels and routine blood indexes. In proteomic analyses, 130 proteins were differentially accumulated in the Se-deficient and control groups. The differentially expressed proteins were annotated mainly as single-organism process, extracellular region, or binding, respectively, and they were highly enriched in the coagulation and complement cascades. Protein-protein interaction analysis showed several important nodal proteins involved in the regulation of binding, cellular biochemical processes, and signal transduction pathways. To our knowledge, this study is the first to comprehensively analyze blood protein changes in the Przewalski's gazelle under Se-deficient conditions, which reveal that this species has developed physiological mechanisms of adaptation in response to Se-deficiency stress. SIGNIFICANCE: The present study is the first to comprehensively analyze alterations in the protein profiles induced by Se deficiency in the blood of the Przewalski's gazelle, showing that Se-deficiency contributed to a significant reduction in blood Se levels and marked changes in blood parameters, which will likely contribute to a better understanding of the molecular mechanisms of the changes in protein abundance in the Przewalski's gazelle in response to Se-deficiency stress.

Keywords: Molecular mechanism; Proteomic analysis; Przewalski's gazelle; Selenium deficiency.