Development of highly water-dispersible complexes between coenzyme Q10 and protein hydrolysates

Asuka Inada; Taishi Oue; Soshi Yamashita; Masao Yamasaki; Tatsuya Oshima; Hideto Matsuyama

doi:10.1016/j.ejps.2019.05.014

Development of highly water-dispersible complexes between coenzyme Q₁₀ and protein hydrolysates

Eur J Pharm Sci. 2019 Aug 1:136:104936. doi: 10.1016/j.ejps.2019.05.014. Epub 2019 May 23.

Authors

Asuka Inada¹, Taishi Oue², Soshi Yamashita², Masao Yamasaki³, Tatsuya Oshima⁴, Hideto Matsuyama⁵

Affiliations

¹ Center for Membrane and Film Technology, Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan. Electronic address: a.inada@port.kobe-u.ac.jp.
² Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan.
³ Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan. Electronic address: myamasaki@cc.miyazaki-u.ac.jp.
⁴ Department of Applied Chemistry, Faculty of Engineering, University of Miyazaki, 1-1 Gakuen Kibanadai Nishi, Miyazaki 889-2192, Japan. Electronic address: oshimat@cc.miyazaki-u.ac.jp.
⁵ Center for Membrane and Film Technology, Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan. Electronic address: matuyama@kobe-u.ac.jp.

PMID: 31129022
DOI: 10.1016/j.ejps.2019.05.014

Abstract

The water-solubility of coenzyme Q₁₀ (CoQ₁₀) is extremely low because of its hydrophobicity, which results in low bioavailability. In this study, peptide mixtures derived from different proteins (casein, albumin, gelatin, lysozyme, zein and hemoglobin) were prepared and used as dispersants ofCoQ₁₀. Most peptide mixtures, except for that derived from lysozyme, enhanced the water-dispersibility of CoQ₁₀ by forming complexes with CoQ₁₀. In particular, the apparent solubility of CoQ₁₀complexed with the casein hydrolysate (Pep_cas) was the highest. Pep_cas was fractionated by ammonium sulfate precipitation and ultrafiltration to find the most effective peptides for enhancing water-dispersibility of CoQ₁₀. The peptide fraction that had a relatively hydrophobic nature and peptides of higher-than-average molecular weights (Pep_casA) was found to be the most effective. Results from differential thermal analysis and powder X-ray diffraction revealed that the complex between CoQ₁₀ and Pep_casA in the solid state was amorphous. In solution, the complex is a hydrocolloid with particle sizes of 154-279 nm.

Keywords: Coenzyme Q(10); Complexation; Dispersant; Hydrocolloid; Peptide.

MeSH terms

Ammonium Sulfate / chemistry
Biological Availability
Caseins / chemistry
Colloids / chemistry
Hydrophobic and Hydrophilic Interactions / drug effects
Molecular Weight
Muramidase / chemistry
Particle Size
Peptides / chemistry
Powders / chemistry
Protein Hydrolysates / chemistry*
Proteins / chemistry
Solubility / drug effects
Ubiquinone / analogs & derivatives*
Ubiquinone / chemistry
Water / chemistry*
X-Ray Diffraction / methods

Substances

Caseins
Colloids
Peptides
Powders
Protein Hydrolysates
Proteins
Water
Ubiquinone
casein hydrolysate
Muramidase
coenzyme Q10
Ammonium Sulfate