Genomic insights into a robust gamma-aminobutyric acid-producer Lactobacillus brevis CD0817

Dandan Gao; Kunpeng Chang; Gongtao Ding; Hongjing Wu; Yuanhong Chen; Mengya Jia; Xiaohua Liu; Shuixing Wang; Yuyuan Jin; Hao Pan; Haixing Li

doi:10.1186/s13568-019-0799-0

Genomic insights into a robust gamma-aminobutyric acid-producer Lactobacillus brevis CD0817

AMB Express. 2019 May 24;9(1):72. doi: 10.1186/s13568-019-0799-0.

Authors

Dandan Gao¹, Kunpeng Chang^{2

3}, Gongtao Ding¹, Hongjing Wu⁴, Yuanhong Chen^{2

3}, Mengya Jia^{2

3}, Xiaohua Liu³, Shuixing Wang³, Yuyuan Jin³, Hao Pan³, Haixing Li^{5

6

7}

Affiliations

¹ Biomedical Research Center, College of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, 730030, Gansu, People's Republic of China.
² State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, Jiangxi, People's Republic of China.
³ Sino-German Joint Research Institute, Nanchang University, Nanchang, 330047, Jiangxi, People's Republic of China.
⁴ Nanchang University College of Science and Technology, Nanchang, 330029, Jiangxi, People's Republic of China.
⁵ Biomedical Research Center, College of Life Sciences and Engineering, Northwest Minzu University, Lanzhou, 730030, Gansu, People's Republic of China. hxli@ncu.edu.cn.
⁶ State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, 330047, Jiangxi, People's Republic of China. hxli@ncu.edu.cn.
⁷ Sino-German Joint Research Institute, Nanchang University, Nanchang, 330047, Jiangxi, People's Republic of China. hxli@ncu.edu.cn.

Abstract

Lactobacillus brevis CD0817, a strain isolated from a healthy adult gut, was currently the most efficient lactic acid bacterial cell factory for gamma-aminobutyric acid. In this study, the complete genome sequence of CD0817 was determined and compared with some related L. brevis genomes. The CD0817 genome consists of one 2,990,570-bp chromosome and four plasmids. The comparative genomic and phylogenetic analysis revealed that L. brevis CD0817 was not very conserved with low GABA-producing L. brevis strains. A significant divergence was that CD0817 harbors only the gadCA operon whereas the low GABA-producing L. brevis strains contain the operon and gadB. The gadB seemed to only marginally contribute to the accumulation of GABA. The high GABA production ability of CD0817 may be associated with its extraordinary genome.

Keywords: Comparative genomics analysis; Complete genome sequence; Gamma-aminobutyric acid; Glutamic acid decarboxylase; Lactobacillus brevis CD0817.

Abstract

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