Nonfunctionalized PNAs as Beacons for Nucleic Acid Detection in a Nanopore System

Alina Asandei; Loredana Mereuta; Jonggwan Park; Chang Ho Seo; Yoonkyung Park; Tudor Luchian

doi:10.1021/acssensors.9b00553

Nonfunctionalized PNAs as Beacons for Nucleic Acid Detection in a Nanopore System

ACS Sens. 2019 Jun 28;4(6):1502-1507. doi: 10.1021/acssensors.9b00553. Epub 2019 May 28.

Authors

Alina Asandei, Loredana Mereuta, Jonggwan Park¹, Chang Ho Seo¹, Yoonkyung Park², Tudor Luchian

Affiliations

¹ Department of Bioinformatics , Kongju National University , Kongju , South Korea , 32588.
² Department of Department of Biomedical Science and Research Center for Proteinaceous Materials (RCPM) , Chosun University , Gwangju , South Korea , 61452.

PMID: 31119934
DOI: 10.1021/acssensors.9b00553

Abstract

In this work, single-channel current recordings were used to selectively detect individual ssDNA strands in the vestibule of the α-hemolysin (α-HL) protein nanopore. The sensing mechanism was based on the detection of the intrinsic topological change of target ssDNA molecules after the hybridization with complementary PNA fragments. The readily distinguishable current signatures of PNA-DNA duplexes reversible association with the α-HL's vestibule, in terms of blockade amplitudes and kinetic features, allows specific detection of nucleic acid hybridization.

Keywords: PNA; electrophysiology; protein nanopore; short ssDNA sensing; single nucleotide substitution.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Toxins / chemistry*
Base Pair Mismatch
DNA, Single-Stranded / analysis*
DNA, Single-Stranded / genetics
Electrophysiology / methods
Hemolysin Proteins / chemistry*
Nanopores*
Nucleic Acid Hybridization
Peptide Nucleic Acids / chemistry*
Peptide Nucleic Acids / genetics
Staphylococcus aureus / chemistry

Substances

Bacterial Toxins
DNA, Single-Stranded
Hemolysin Proteins
Peptide Nucleic Acids
staphylococcal alpha-toxin