Biohydrogen and polyhydroxyalkanoate production from original hydrolyzed polyacrylamide-containing wastewater

Lanmei Zhao; Dong Han; Zichao Yin; Mutai Bao; Jinren Lu

doi:10.1016/j.biortech.2019.121404

Biohydrogen and polyhydroxyalkanoate production from original hydrolyzed polyacrylamide-containing wastewater

Bioresour Technol. 2019 Sep:287:121404. doi: 10.1016/j.biortech.2019.121404. Epub 2019 May 13.

Authors

Lanmei Zhao¹, Dong Han², Zichao Yin¹, Mutai Bao³, Jinren Lu⁴

Affiliations

¹ Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education/Institute for Advanced Ocean Study, Ocean University of China, Qingdao 266100, China; College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China.
² College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
³ Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education/Institute for Advanced Ocean Study, Ocean University of China, Qingdao 266100, China; College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China. Electronic address: mtbao@ouc.edu.cn.
⁴ College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China.

PMID: 31108414
DOI: 10.1016/j.biortech.2019.121404

Abstract

This work aimed to study biohydrogen (H₂) and polyhydroxyalkanoate (PHA) production from original hydrolyzed polyacrylamide (HPAM)-containing wastewater. NH₄⁺-N from HPAM hydrolysis was removed efficiently through short-cut nitrification and anoxic ammonia oxidation (anammox). Carbon/Nitrogen (C/N) ratios of effluent reached 51-97, and TOC decreased only 2%-4%, providing potential for subsequent H₂ and PHA production. The maximum yields of H₂ (0.833 mL·mg^-1_substrate) and Volatile Fatty Acid (VFA) (465 mg·L^-1) occurred at influent C/N ratio of 51. Substrate removal increased linearly with the activities of dehydrogenase and hydrogenase (R² ≥ 0.990), and H₂ yield rose exponentially with enzyme activities (R² ≥ 0.989). The maximum PHA yield (54.2% VSS) occurred at the 42nd hour and influent C/N ratio of 97. PHA yield was positively correlated with substrate uptake. The change of H₂-producing, PHA-accumulating and HPAM-degradating bacteria indicated that those functional microorganisms had synergistic effects on H₂ production and substrate uptake, as well as PHA accumulation and substrate uptake.

Keywords: Carbon/nitrogen ratio; Enzyme activities; Functional microorganisms; Hydrogen; Hydrolyzed polyacrylamide; Polyhydroxyalkanoate.

MeSH terms

Acrylic Resins
Bioreactors
Nitrification
Polyhydroxyalkanoates*
Wastewater*

Substances

Acrylic Resins
Polyhydroxyalkanoates
Waste Water
polyacrylamide