Self-healing adhesive with antibacterial activity in water-aging for 12 months

Junling Wu; Chuanjian Zhou; Jianping Ruan; Michael D Weir; Franklin Tay; Jirun Sun; Mary Anne S Melo; Thomas W Oates; Xiaofeng Chang; Hockin H K Xu

doi:10.1016/j.dental.2019.05.004

Self-healing adhesive with antibacterial activity in water-aging for 12 months

Dent Mater. 2019 Aug;35(8):1104-1116. doi: 10.1016/j.dental.2019.05.004. Epub 2019 May 16.

Authors

Junling Wu¹, Chuanjian Zhou², Jianping Ruan³, Michael D Weir⁴, Franklin Tay⁵, Jirun Sun⁶, Mary Anne S Melo⁴, Thomas W Oates⁴, Xiaofeng Chang⁷, Hockin H K Xu⁸

Affiliations

¹ Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Department of Prosthodontics, School of Stomatology, Shandong University, Jinan 250012, China. Electronic address: doctorwujunling@163.com.
² Research Institute of Polymer Materials, School of Materials Science and Engineering, Shandong University, Jinan 250061, China.
³ Clinical Research Center of Shanxi Province for Dental and Maxillofacial Diseases, Key Laboratory of Shanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shanxi 710004, China.
⁴ Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA.
⁵ Department of Endodontics, Dental College of Georgia, Augusta University, Augusta, GA, USA.
⁶ Volpe Research Center, American Dental Association Foundation, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
⁷ Clinical Research Center of Shanxi Province for Dental and Maxillofacial Diseases, Key Laboratory of Shanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shanxi 710004, China. Electronic address: changxf@xjtu.edu.cn.
⁸ Department of Advanced Oral Sciences & Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USA; Center for Stem Cell Biology & Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA; Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA. Electronic address: hxu@umaryland.edu.

PMID: 31104922
DOI: 10.1016/j.dental.2019.05.004

Abstract

Objective: Secondary caries and micro-cracks are the main limiting factors for dentin bond durability. The objectives of this study were to develop a self-healing adhesive containing dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP), and investigate the effects of water-aging for 12 months on self-healing, dentin bonding, and antibacterial properties for the first time.

Methods: Microcapsules were synthesized with poly (urea-formaldehyde) (PUF) shells containing triethylene glycol dimethacrylate (TEGDMA) and N,N-dihydroxyethyl-p-toluidine (DHEPT). The adhesive contained 7.5% microcapsules, 10% DMAHDM, and 20% NACP (all mass). Specimens were water-aged at 37 °C for 1 day to 12 months. Dentin bond strength was measured using extracted human teeth. A single-edge-V-notched-beam (SEVNB) method was used to measure fracture toughness K_IC and self-healing efficiency. A dental plaque microcosm biofilm model was used with human saliva as inoculum.

Results: The microcapsules + DMAHDM + NACP group showed no decline in dentin bond strength after water-aging for 12 months, which was significantly higher than that of other groups without DMAHDM (p < 0.05). A self-healing efficiency of 67% recovery in K_IC was obtained even after 12 months of water immersion, indicating that the self-healing ability was not lost in water-aging (p > 0.1). The bacteria-killing ability of this adhesive did not decline from 1 day to 12 months (p > 0.1), with biofilm CFU reduction by 3-4 orders of magnitude after the resin was water-aged for 12 months, compared to control resin.

Significance: This novel adhesive with triple merits of self-healing, antibacterial and remineralization functions showed an excellent long-term durability in water-aging for 12 months. This multifunctional adhesive has the potential for dental applications to heal cracks, inhibit bacteria, provide ions for remineralization, and increase the restoration longevity.

Keywords: Antimicrobial; Dental adhesive; Microcapsules; Remineralization; Self-healing; Water-aging.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents
Biofilms
Child, Preschool
Dental Cements*
Humans
Infant
Methacrylates
Water*

Substances

Anti-Bacterial Agents
Dental Cements
Methacrylates
Water