Video-based assessments of the hind limb stepping in a mouse model of hemi-parkinsonism

Masatoshi Ogawa; Yu Zhou; Ryosuke Tsuji; Satoshi Goto; Jiro Kasahara

doi:10.1016/j.neures.2019.05.002

Video-based assessments of the hind limb stepping in a mouse model of hemi-parkinsonism

Neurosci Res. 2020 May:154:56-59. doi: 10.1016/j.neures.2019.05.002. Epub 2019 May 16.

Authors

Masatoshi Ogawa¹, Yu Zhou¹, Ryosuke Tsuji², Satoshi Goto³, Jiro Kasahara⁴

Affiliations

¹ Department of Neurobiology and Therapeutics, Institute of Biomedical Sciences, Graduate School of Pharmaceutical Sciences, Tokushima University, Tokushima 770-8505, Japan; Department of Neurodegenerative Disorders Research, Institute of Biomedical Sciences, Graduate School of Medical Sciences, Tokushima University, Tokushima 770-8503, Japan.
² Department of Neurobiology and Therapeutics, Institute of Biomedical Sciences, Graduate School of Pharmaceutical Sciences, Tokushima University, Tokushima 770-8505, Japan.
³ Department of Neurodegenerative Disorders Research, Institute of Biomedical Sciences, Graduate School of Medical Sciences, Tokushima University, Tokushima 770-8503, Japan; Parkinson's Disease and Dystonia Research Center, Tokushima University Hospital, Tokushima 770-8503, Japan.
⁴ Department of Neurobiology and Therapeutics, Institute of Biomedical Sciences, Graduate School of Pharmaceutical Sciences, Tokushima University, Tokushima 770-8505, Japan. Electronic address: awajiro@tokushima-u.ac.jp.

PMID: 31103423
DOI: 10.1016/j.neures.2019.05.002

Abstract

Unilateral injection of 6-hydroxydopamine (6-OHDA) is commonly used to generate a rodent model of Parkinson's disease (PD). Although motor deficits of the lower extremities represent one of the major clinical symptoms in PD patients, validated tests for assessing motor impairments of the hind limb in 6-OHDA mice are currently unavailable. We here report the video-based assessments of the asymmetric use of hind limbs in 6-OHDA mice. A significantly decreased number of spontaneous hind limb stepping was observed in the contralateral-to-lesioned side, and was dose dependently reversed by levodopa, suggesting that it could be utilized for screening PD therapeutics.

Keywords: 6-OHDA; Hemi-parkinsonism; Hind limb stepping; Mouse.

MeSH terms

Animals
Corpus Striatum / metabolism
Disease Models, Animal*
Gait / drug effects*
Hindlimb
Levodopa / pharmacology
Male
Mice
Mice, Inbred C57BL
Oxidopamine
Parkinsonian Disorders / chemically induced*
Tyrosine 3-Monooxygenase / metabolism

Substances

Levodopa
Oxidopamine
Tyrosine 3-Monooxygenase