Structure and allosteric coupling of type Ⅱ antitoxin CopASO

Rongjuan Zhao; Quan Li; Jingjing Zhang; Fudong Li; Jianyun Yao; Jiahai Zhang; Lin Liu; Xiaoxue Wang; Xuecheng Zhang

doi:10.1016/j.bbrc.2019.05.049

Structure and allosteric coupling of type Ⅱ antitoxin CopA_SO

Biochem Biophys Res Commun. 2019 Jul 5;514(4):1122-1127. doi: 10.1016/j.bbrc.2019.05.049. Epub 2019 May 14.

Authors

Rongjuan Zhao¹, Quan Li¹, Jingjing Zhang¹, Fudong Li², Jianyun Yao³, Jiahai Zhang², Lin Liu¹, Xiaoxue Wang⁴, Xuecheng Zhang⁵

Affiliations

¹ School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui, 230601, China; Anhui Key Laboratory of Modern Biomanufacturing, Hefei, Anhui, 230601, China.
² School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, China.
³ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, Guangdong, 510301, China.
⁴ CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Institution of South China Sea Ecology and Environmental Engineering, Chinese Academy of Sciences, Guangzhou, Guangdong, 510301, China. Electronic address: xxwang@scsio.ac.cn.
⁵ School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China; Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, Anhui, 230601, China; Anhui Key Laboratory of Modern Biomanufacturing, Hefei, Anhui, 230601, China. Electronic address: turenzh@ahu.edu.cn.

PMID: 31101334
DOI: 10.1016/j.bbrc.2019.05.049

Abstract

Toxin-antitoxin (TA) systems play critical roles in the environment adaptation of bacteria. Allosteric coupling between the N-terminal DNA-binding domain and the C-terminal toxin-binding domain of antitoxins contributes to conditional cooperativity in the functioning of type II TA. Herein, using circular dichroism (CD), nuclear magnetic resonance (NMR), X-ray crystallography, and size exclusion chromatography (SEC), the structure and DNA binding of CopA_SO, a newly identified type II antitoxin in Shewanella oneidensis, were investigated. Our data show that CopA_SO is a typical RHH antitoxin with an ordered N-terminal domain and a disordered C-terminal domain, and furthermore indicate that the C-terminal domain facilitates DNA binding of the N-terminal domain, which in turn induces the C-terminal domain to fold and associate.

Keywords: Allosteric coupling; Antitoxin; CopA(SO); Ribbon-helix-helix motif; Shewanella oneidensis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Allosteric Regulation
Antitoxins / chemistry*
Antitoxins / metabolism*
Circular Dichroism
Crystallography, X-Ray
DNA / chemistry
DNA / metabolism
Models, Molecular
Nuclear Magnetic Resonance, Biomolecular
Shewanella / chemistry*

Substances

Antitoxins
DNA

Supplementary concepts

Shewanella oneidensis