Profile analysis reveals transfer RNA fragments involved in mesangial cells proliferation

Xiaoyu Lu; Xianyi Zhu; Minyi Yu; Chen Na; Weihua Gan; Aiqing Zhang

doi:10.1016/j.bbrc.2019.04.022

Profile analysis reveals transfer RNA fragments involved in mesangial cells proliferation

Biochem Biophys Res Commun. 2019 Jul 5;514(4):1101-1107. doi: 10.1016/j.bbrc.2019.04.022. Epub 2019 May 13.

Authors

Xiaoyu Lu¹, Xianyi Zhu¹, Minyi Yu¹, Chen Na², Weihua Gan³, Aiqing Zhang¹

Affiliations

¹ Department of Pediatric Nephrology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, China.
² Department of Pediatrics, Yanan Hospital Affiliated to Kunming Medicical University, Kunming, 650051, China.
³ Department of Pediatric Nephrology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, 210003, China. Electronic address: weihuagan@njmu.edu.cn.

PMID: 31097225
DOI: 10.1016/j.bbrc.2019.04.022

Abstract

Mesangial cell (MCs) proliferation is an essential component of glomerulonephritis. To find some bio-markers of mesangial cell proliferation, we investigate the relationship between transfer RNA fragments (tRFs) and proliferating mesangial cells. The model of proliferating mesangial cells was built by using transforming growth factor-1(TGF-β1) treated mesangial cells. Then we analyzed the expression of tRFs in normal mesangial cells and mesangial cells treated by TGF-β1 through high-throughput sequencing technique. qRT-PCR was conducted to validate the differently expressed tRFs in normal mesangial cells and mesangial cells treated by TGF-β1. tDR-000064 and tDR-000103 were notably down-regulated in mesangial cells treated by TGF-β1 compared with normal mesangial cells. Then we confirmed that tDR-000064 and tDR-000103 were correlated with proliferation of mesangial cells through receiver operating characteristic curve analysis. Furthermore, Gene ontology (GO) and pathway analysis demonstrated that the two dys-regulated tRFs were mostly involved in mesangial cells and TGF-β1 receptor-mediated signaling pathway. Our research provides a comprehensive analysis of tRFs in proliferating mesangial cells. (Figure 1A).

Keywords: Mesangial cells; Proliferation; Transfer RNA fragments.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Proliferation
Cell Survival
Cells, Cultured
Gene Expression Profiling
Glomerulosclerosis, Focal Segmental / metabolism
Mesangial Cells / cytology*
Mesangial Cells / metabolism
RNA, Transfer / genetics*
RNA, Transfer / isolation & purification
RNA, Transfer / metabolism
Rats
Transforming Growth Factor beta1 / metabolism*

Substances

Tgfb1 protein, rat
Transforming Growth Factor beta1
RNA, Transfer