Effect of acid treatment on extraction yield and gel strength of gelatin from whiptail stingray (Dasyatis brevis) skin

Marco Antonio Sántiz-Gómez; Miguel Angel Mazorra-Manzano; Hugo Enrique Ramírez-Guerra; Susana María Scheuren-Acevedo; Gerardo Navarro-García; Ramón Pacheco-Aguilar; Juan Carlos Ramírez-Suárez

doi:10.1007/s10068-018-0514-y

Effect of acid treatment on extraction yield and gel strength of gelatin from whiptail stingray (Dasyatis brevis) skin

Food Sci Biotechnol. 2018 Nov 24;28(3):751-757. doi: 10.1007/s10068-018-0514-y. eCollection 2019 Jun.

Authors

Marco Antonio Sántiz-Gómez¹, Miguel Angel Mazorra-Manzano¹, Hugo Enrique Ramírez-Guerra¹, Susana María Scheuren-Acevedo¹, Gerardo Navarro-García², Ramón Pacheco-Aguilar¹, Juan Carlos Ramírez-Suárez¹

Affiliations

¹ 1Centro de Investigación en Alimentación y Desarrollo, A.C. Carretera a la Victoria Km 0.6. Apdo, Postal 1735, C.P. 83000 Hermosillo, Sonora Mexico.
² 2Departamento de Investigaciones Científicas y Tecnológicas, Universidad de Sonora, Hermosillo, Sonora Mexico.

Abstract

Chemical properties of fish gelatins differ from those of conventional mammalian sources, representing an attractive technological alternative for the food industry. Ray filleting generates a considerable amount of skin waste that can be used as a collagen source for gelatin extraction. Thus, this research evaluated the HCl and CH₃COOH effect, at 0.01, 0.025, 0.05, 0.075, 0.1, 0.15, and 0.2 M, on extraction yield, molecular weight distribution, and gel strength (GS) of whiptail stingray (Dasyatis brevis) skin gelatins. Results showed differences (P < 0.05) between acid type and concentration used. CH₃COOH (0.15 M) gave the highest extraction yield (7.0% vs. 5.5% at 0.15 M HCl) and GS (653 ± 71 g vs. 619.5 ± 82 g at 0.2 M HCl). Gelatin electrophoretic profile from CH₃COOH revealed α-/β-components and high molecular weight (> 200 kDa) polymers. Ray gelatin GS was higher than commercial bovine gelatin, suggesting its possible use for technological food applications.

Keywords: Acid treatment; Extraction yield; Fish gelatin; Fish skin collagen; Gel strength.