Post-genomic approach based discovery of alkylresorcinols from a cricket-associated fungus, Penicillium soppi

Org Biomol Chem. 2019 May 29;17(21):5239-5243. doi: 10.1039/c9ob00807a.

Abstract

Polyketide synthase (PKS) gene-guided genome mining in a cricket-associated fungus, Penicillium soppi, revealed a cryptic biosynthetic gene cluster that contained a highly reducing PKS (HR-PKS), a type III PKS, and a P450 gene. Heterologous expression of the cluster in Aspergillus oryzae led to the isolation of novel alkylresorcinols with a unique Z,E,Z-triene motif. This study displays an unusual biosynthetic mechanism of an HR-PKS and a new releasing mechanism via a type III PKS in fungi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Drug Discovery*
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / isolation & purification
  • Enzyme Inhibitors / pharmacology*
  • Molecular Structure
  • Penicillium / chemistry*
  • Polyketide Synthases / antagonists & inhibitors*
  • Polyketide Synthases / metabolism
  • Resorcinols / chemistry
  • Resorcinols / isolation & purification
  • Resorcinols / pharmacology*

Substances

  • Enzyme Inhibitors
  • Resorcinols
  • Polyketide Synthases