Massive volumetric imaging of cleared tissue: The necessary tools to be successful

Alan M Watson; Simon C Watkins

doi:10.1016/j.biocel.2019.05.007

Massive volumetric imaging of cleared tissue: The necessary tools to be successful

Int J Biochem Cell Biol. 2019 Jul:112:76-78. doi: 10.1016/j.biocel.2019.05.007. Epub 2019 May 11.

Authors

Alan M Watson¹, Simon C Watkins²

Affiliations

¹ The Department of Cell Biology and The Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: alan.watson@pitt.edu.
² The Department of Cell Biology and The Center for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA, USA.

PMID: 31085331
DOI: 10.1016/j.biocel.2019.05.007

Abstract

Recent technological innovations in high-speed microscopes, tissue clearing methods, optics and computation have made it possible to image enormous biologic volumes. These techniques are applicable to a broad range of biomedical disciplines and are becoming a necessary tool for exploring biologic systems that extend beyond cellular microenvironments to entire organs and organisms. Here we broadly discuss the tool sets that are available for cleared tissue imaging and how various choices may influence the planning and feasibility of an experiment.

Keywords: Big data; Clarity; Tissue clearing; Volumetric imaging; Whole animal imaging; Whole organ imaging.

Publication types

Review

MeSH terms

Animals
Cell Size*
Humans
Optical Imaging* / instrumentation
Optical Imaging* / methods