Hepatoprotective steroids from roots of Cynanchum otophyllum

Jinrun Dong; Xingrong Peng; Shuangyang Lu; Lin Zhou; Minghua Qiu

doi:10.1016/j.fitote.2019.104171

Hepatoprotective steroids from roots of Cynanchum otophyllum

Fitoterapia. 2019 Jul:136:104171. doi: 10.1016/j.fitote.2019.104171. Epub 2019 May 11.

Authors

Jinrun Dong¹, Xingrong Peng², Shuangyang Lu¹, Lin Zhou², Minghua Qiu³

Affiliations

¹ State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, China; University of the Chinese Academy of Sciences, Beijing, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
² State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
³ State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China. Electronic address: mhchiu@mail.kib.ac.cn.

PMID: 31085309
DOI: 10.1016/j.fitote.2019.104171

Abstract

Seven undescribed C21 steroids, namely cynanchin A-G, together with thirteen known analogues, were isolated from the roots of cynanchum otophyllum. Their structures were elucidated by 1D, 2D NMR and MS spectra, as well as chemical methods. Meanwhile, all of isolates were tested for their anti-hepatic fibrosis activity. Among them, compounds 4-6, 10-12 and 14-17 showed moderate or significant inhibitory effects for the proliferation of hepatic stellate cells (HSCs) induced by transforming growth factor-β1 (TGF-β1) in vitro.

Keywords: Anti-liver fibrosis activity; Asclepiadaceae; Cynanchum otophyllum; Steriods.

MeSH terms

Cell Line
Cell Proliferation / drug effects
China
Cynanchum / chemistry*
Hepatic Stellate Cells / drug effects*
Humans
Molecular Structure
Phytochemicals / isolation & purification
Phytochemicals / pharmacology
Plant Roots / chemistry*
Steroids / isolation & purification
Steroids / pharmacology*
Transforming Growth Factor beta1

Substances

Phytochemicals
Steroids
Transforming Growth Factor beta1