Balance between nitrogen use efficiency and cadmium tolerance in Brassica napus and Arabidopsis thaliana

Abstract

The transmembrane transport of NO₃^- and Cd²⁺ into plant cell vacuoles relies on the energy from their tonoplast proton pumps, V-ATPase and V-PPase. If the activity of these pumps is reduced, it results in less NO₃^- and Cd²⁺ being transported into the vacuoles, which contributes to better nitrogen use efficiency (NUE) and lower Cd²⁺ tolerance in plants. The physiological mechanisms that regulate the balance between NUE and Cd²⁺ tolerance remain unknown. In our study, two Brassica napus genotypes with differential NUEs, xiangyou 15 and 814, and Atclca-2 mutant and AtCAX4 over-expression line (AtCAX4-OE) of Arabidopsis thaliana, were used to investigate Cd²⁺ stress responses. We found that the Brassica napus genotype, with higher NUE, was more sensitive to Cd²⁺ stress. The AtCAX4-OE mutant, with higher Cd²⁺ vacuolar sequestration capacity (VSC), limited NO₃^- sequestration into root vacuoles and promoted NUE. Atclca-2 mutants, with decreased NO₃^- VSC, enhanced Cd²⁺ sequestration into root vacuoles and conferred greater Cd²⁺ tolerance than the WT. This may be due to the competition between Cd²⁺ andNO₃^- in the vacuoles for the energy provided by V-ATPase and V-PPase. Regulating the balance between Cd²⁺ and NO₃^- vacuolar accumulation by inhibiting the activity of CLCa transporter and increasing the activity of CAX4 transporter will simultaneously enhance both the NUE and Cd²⁺ tolerance of Brassica napus, essential for improving its Cd²⁺ phytoremediation potential.

Keywords: Heavy metal tolerance; Nitrate assimilation; Nitrogen use efficiency; Proton pumps; Vacuolar sequestration capacity (VSC).