Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi

Aline Lamien-Meda; Hans-Peter Fuehrer; Harald Noedl

doi:10.1016/j.dib.2019.103937

Data on High Resolution Melting (HRM) and phylogenetic analysis of P. ovale wallikeri and P. ovale curtisi

Data Brief. 2019 Apr 23:24:103937. doi: 10.1016/j.dib.2019.103937. eCollection 2019 Jun.

Authors

Aline Lamien-Meda¹, Hans-Peter Fuehrer², Harald Noedl¹

Affiliations

¹ Institute of Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Austria.
² Institute of Parasitology, University of Veterinary Medicine, Vienna, Austria.

Abstract

High Resolution Melting (HRM) analysis is a post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. These data are presenting the identity of the 33 samples used for a qPCR-HRM and a nested snapback methods validation. In addition we are presenting the high resolution melting profiles of P. ovale curtisi (Poc) and P. ovale wallikeri (Pow) in the following conditions: after a direct qPCR run and after a nested snapback run. The qPCR-HRM of artificial mixture of Poc and Pow plasmids (200 copies/μl, each) at different proportions are showing the melting pattern of co-infections with both species. The sequencing methodology of the clpc gene fragment of 12 randomly selected samples is described and their likeness to published sequences is shown in a maximum likelihood tree. "Novel high resolution melting and snapback assays for simultaneous detection and differentiation of Plamodium ovale spp." [1].