Evaluation, comparison of different solvent extraction, cell disruption methods and hydrothermal liquefaction of Oedogonium macroalgae for biofuel production

Bharti Ramola; Vinod Kumar; Manisha Nanda; Yashi Mishra; Tushar Tyagi; Ayushi Gupta; Nishesh Sharma

doi:10.1016/j.btre.2019.e00340

Evaluation, comparison of different solvent extraction, cell disruption methods and hydrothermal liquefaction of Oedogonium macroalgae for biofuel production

Biotechnol Rep (Amst). 2019 Apr 22:22:e00340. doi: 10.1016/j.btre.2019.e00340. eCollection 2019 Jun.

Authors

Bharti Ramola¹, Vinod Kumar¹, Manisha Nanda², Yashi Mishra¹, Tushar Tyagi¹, Ayushi Gupta³, Nishesh Sharma³

Affiliations

¹ Department of Chemistry, Uttaranchal University, Dehradun, 248007, India.
² Department of Biotechnology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Dehradun, 248007, India.
³ Department of Biotechnology, Uttaranchal University, Dehradun, 248007, India.

Abstract

Cell disruption and lipid extraction methods for macroalgae are not well reported. Therefore, we compared various lipid extraction methods and extraction efficiency of various solvents to improve lipid yields from Oedogonium fresh water macroalgae. Lipid extraction was done by 2 methods viz., modified Bligh and Dyer method and soxhlet extraction using either single solvents or mixtures. In soxhlet extraction method five solvents were used (1) Hexane commonly used solvent for lipid extractions, (2) chloroform: methanol (2:1), (3) Chloroform: hexane (1:1), (4) Chloroform: hexane (1:2), (5) Dichloromethane + methanol (2:1). To improve lipid extraction yields, various cell disruption methods were also compared during the present study. Impurities of chlorophyll and protein were also detected in the extracted lipids. Hydrothermal liquefaction of algal biomass with TiO₂ was also conducted at 300 °C. HTL was more effective by which 23.3 wt% of bio-crude oil was obtained.

Keywords: Biodiesel; Cell disruption; HTL; Lipid; Macroalgae; Oedogonium.