The nitric oxide suppressed Arabidopsis mutants- Atnoa1 and Atnia1nia2noa1-2 produce nitric oxide in MS growth medium and on uranium exposure

Rajesh Kumar Tewari; Nele Horemans; Robin Nauts; Jean Wannijn; May Van Hees; Hildegarde Vandenhove

doi:10.1016/j.plaphy.2019.04.042

The nitric oxide suppressed Arabidopsis mutants- Atnoa1 and Atnia1nia2noa1-2 produce nitric oxide in MS growth medium and on uranium exposure

Plant Physiol Biochem. 2019 Jul:140:9-17. doi: 10.1016/j.plaphy.2019.04.042. Epub 2019 May 2.

Authors

Rajesh Kumar Tewari¹, Nele Horemans², Robin Nauts³, Jean Wannijn⁴, May Van Hees⁵, Hildegarde Vandenhove⁶

Affiliations

¹ Department of Botany, University of Lucknow, Lucknow, 226007, India; Biosphere Impact Studies, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, Mol, 2400, Belgium. Electronic address: rktewari_bot@yahoo.com.
² Biosphere Impact Studies, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, Mol, 2400, Belgium; Hasselt University, Centre for Environmental Sciences, Agoralaan Building D, 3590, Diepenbeek, Belgium. Electronic address: nhoreman@sckcen.be.
³ Department of Botany, University of Lucknow, Lucknow, 226007, India. Electronic address: rnauts@sckcen.be.
⁴ Biosphere Impact Studies, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, Mol, 2400, Belgium. Electronic address: jwannijg@sckcen.be.
⁵ Biosphere Impact Studies, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, Mol, 2400, Belgium. Electronic address: mvhees@sckcen.be.
⁶ Biosphere Impact Studies, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, Mol, 2400, Belgium. Electronic address: hvandenh@sckcen.be.

PMID: 31078053
DOI: 10.1016/j.plaphy.2019.04.042

Abstract

The mutants Atnoa1 and Atnia1nia2noa1-2 having a defective chloroplast developmental process, showed enhanced chlorophyll levels when they were grown on Murashige and Skoog (MS) medium and on exposure with uranium (U) on Hoagland medium. Thus we hypothesized that these mutants probably produced NO in MS medium and on exposure with U. Wild-type Col-0, Atnoa1, Atnia1nia2noa1-2 plants were cultured on modified Hoagland and 1/10 MS media and NO generation in the roots of these mutants was monitored using NO selective fluorescent dyes, DAF-2DA and Fl2E. Both Atnoa1 and Atnia1nia2noa1-2 triple mutants produced NO as observed by increases in DAF-2T and Fl2E fluorescence when these mutants were grown on MS medium but not on Hoagland medium. In presence of NO scavenger, methylene blue (MB, 200 μM), DAF-2T and Fl2E fluorescence was completely abolished. On the other hand treatment of the plants with 25 μM U triggered NO generation. U-treated Atnoa1 and Atnia1nia2noa1-2 plants upregulated genes (POR B, POR D, CHL D) involved in the chlorophyll biosynthesis. From these results it was concluded that Atnoa1 and Atnia1nia2noa1-2 are conditional NO producers and it appears that NO generation in plants substantially depends on growth medium and NIA1, NIA2 or NOA1 does not appear to be really involved in NO generation in MS medium or after U exposure.

Keywords: Atnia1nia2noa1-2; Atnoa1; Hoagland medium; MS medium; Nitric oxide; Uranium.

MeSH terms

Arabidopsis / drug effects
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / drug effects
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism
Gene Expression Regulation, Plant / drug effects
Gene Expression Regulation, Plant / genetics
Mutation / genetics
Nitric Oxide / pharmacology*
Uranium / pharmacology

Substances

Arabidopsis Proteins
Nitric Oxide
Uranium