A divergent canine parvovirus type 2c (CPV-2c) isolate circulating in China

Chuanfeng Li; Jingyu Tang; Zongyan Chen; Guangbin Niu; Guangqing Liu

doi:10.1016/j.meegid.2019.05.004

A divergent canine parvovirus type 2c (CPV-2c) isolate circulating in China

Infect Genet Evol. 2019 Sep:73:242-247. doi: 10.1016/j.meegid.2019.05.004. Epub 2019 May 8.

Authors

Chuanfeng Li¹, Jingyu Tang², Zongyan Chen², Guangbin Niu³, Guangqing Liu⁴

Affiliations

¹ Innovation Team of Small Animal Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences(CAAS), Shanghai 200241, China. Electronic address: lichuanfeng@shvri.ac.cn.
² Innovation Team of Small Animal Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences(CAAS), Shanghai 200241, China.
³ Shanghai Center for Animal Disease Control and Prevention, Shanghai 201103, China.
⁴ Innovation Team of Small Animal Infectious Disease, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences(CAAS), Shanghai 200241, China. Electronic address: liugq@shvri.ac.cn.

PMID: 31077840
DOI: 10.1016/j.meegid.2019.05.004

Abstract

The variability and the intrinsically high mutation rate of canine parvovirus type 2(CPV-2) increased the diversity of CPV-2 in canine populations. Since the first occurrence of CPV-2, three antigenic variants (2a, 2b and 2c) were detected and distributed worldwide. CPV-2c infection has been detected and increasingly reported in China. Here, a CPV-2c strain CPV-SH1516 was isolated and its complete genome sequence was first characterized. Compared with other CPV-2c isolates, CPV-2c isolates from China continued to evolve into divergent CPV-2c variants with specific unique amino acid substitutions under purifying selection. Emergence of CPV-2c isolates from China was driven by the unique gradual point mutations in key sites of VP2 rather than introduction from outside China. Combining sequence comparison with phylogenetic analysis based on the amino acid sequences of VP2, the vast majority of CPV-2c isolates from China formed a monophyletic cluster and CPV-SH1516 was a representative isolate of CPV-2c circulating in China. Overall, our study provides valuable insight into the evolutionary mechanism of CPV-2c.

Keywords: Canine parvovirus; Genomic characterization; Phylogenetic analysis; Purifying selection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
China / epidemiology
Dog Diseases / epidemiology
Dog Diseases / virology*
Dogs
Parvoviridae Infections / epidemiology
Parvoviridae Infections / veterinary*
Parvoviridae Infections / virology
Parvovirus, Canine / classification*
Parvovirus, Canine / genetics