Anterior Cruciate Ligament Reconstruction in a Rabbit Model Using a Decellularized Allogenic Semitendinous Tendon Combined with Autologous Bone Marrow-Derived Mesenchymal Stem Cells

Stem Cells Transl Med. 2019 Sep;8(9):971-982. doi: 10.1002/sctm.18-0132. Epub 2019 May 11.

Abstract

As a regular adoptable material for anterior cruciate ligament (ACL) reconstruction, free tendon allograft exhibits unsatisfactory outcomes, such as retarded ligamentization and tendon-bone integration. The application of bone marrow-derived mesenchymal stem cells (BMSCs), as well as a decellularized free tendon allograft developed by our group, was proven to be effective in improving ACL reconstruction results. This study aimed to investigate the efficacy and feasibility of decellularized allogenic semitendinous tendon (ST) combined with autologous BMSCs used as a substitute to free tendon allograft in a rabbit model. This study finally shows that the decellularized allogenic ST combined with autologous BMSCs could significantly improve ACL reconstruction results compared with allograft. Stem Cells Translational Medicine 2019;8:971&982.

Keywords: Anterior cruciate ligament; Bone marrow-derived mesenchymal stem cells; Decellularized allograft; Semitendinous tendon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anterior Cruciate Ligament Injuries / pathology
  • Anterior Cruciate Ligament Injuries / therapy*
  • Bone Density
  • Bone Marrow Cells / cytology
  • Bone and Bones / diagnostic imaging
  • Bone and Bones / pathology
  • Cell Differentiation
  • Cell Lineage
  • Collagen / genetics
  • Collagen / metabolism
  • Hamstring Tendons / transplantation*
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / cytology
  • Rabbits
  • Transplantation, Autologous
  • X-Ray Microtomography

Substances

  • Collagen