Insights into the effect of nickel (Ni(II)) on the performance, microbial enzymatic activity and extracellular polymeric substances of activated sludge

Abstract

The performance, nitrogen removal rate, microbial enzymatic activity and extracellular polymeric substances (EPS) of activated sludge were assessed under nickel (Ni(II)) stress. The organic matter and NH₄⁺-N removal efficiencies were stable at less than 10 mg/L Ni(II) and subsequently decreased with the increment of Ni(II) concentration from 10 to 30 mg/L. The specific oxygen uptake rate and dehydrogenase activity kept stable at less than 5 mg/L Ni(II) and then declined at 5-30 mg/L Ni(II). Both specific ammonia-oxidizing rate (SAOR) and specific nitrite-oxidizing rate (SNOR) decreased with the increment of Ni(II) concentration. The changing trends of ammonia monooxygenase and nitrite oxidoreductase activities were matched those of SAOR and SNOR, respectively. The nitrite-reducing rate and nitrate-reducing rate illustrated a similar variation tendency to the nitrite reductase activity and nitrate reductase activity, respectively. Ni(II) impacted on the production, chemical composition and functional group of EPS. The relation between the sludge volume index and the EPS production exhibited a better linear function with a negative slope, demonstrating that Ni(II) improved the sludge settleability despite of the increase of EPS production.

Keywords: Denitrification rate; Extracellular polymeric substances; Microbial enzymatic activity; Nickel; Nitrification rate.