Phenolic composition, antioxidant and antibacterial properties, and in vitro anti-HepG2 cell activities of wild apricot (Armeniaca Sibirica L. Lam) kernel skins

Fangling Qin; Lu Yao; Cairui Lu; Cong Li; Yanyan Zhou; Chencan Su; Bang Chen; Yehua Shen

doi:10.1016/j.fct.2019.05.007

Phenolic composition, antioxidant and antibacterial properties, and in vitro anti-HepG2 cell activities of wild apricot (Armeniaca Sibirica L. Lam) kernel skins

Food Chem Toxicol. 2019 Jul:129:354-364. doi: 10.1016/j.fct.2019.05.007. Epub 2019 May 6.

Authors

Fangling Qin¹, Lu Yao², Cairui Lu³, Cong Li⁴, Yanyan Zhou⁵, Chencan Su⁶, Bang Chen⁷, Yehua Shen⁸

Affiliations

¹ Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi, 710127, China; College of Chemistry and Chemical Engineering, Shaanxi Province Key Laboratory of Environmental Pollution Control and Reservoir Protection Technology of Oilfields, Xi'an Shiyou University, Xi'an, Shaanxi, 710065, China. Electronic address: flqin@xsyu.edu.cn.
² Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi, 710127, China. Electronic address: hsd19921226@163.com.
³ Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi, 710127, China. Electronic address: lcr2624120008@163.com.
⁴ Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi, 710127, China. Electronic address: licong@nwu.edu.cn.
⁵ Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi, 710127, China. Electronic address: 13071057712@163.com.
⁶ Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi, 710127, China. Electronic address: 1004425512@qq.com.
⁷ Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi, 710127, China. Electronic address: chenbang@nwu.edn.cn.
⁸ Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, Xi'an, Shaanxi, 710127, China. Electronic address: yhshen@nwu.edu.cn.

PMID: 31071388
DOI: 10.1016/j.fct.2019.05.007

Abstract

The polyphenols profiles of the methanol extracts of bitter apricot [Armeniaca Sibirica (L.)] kernel skins (AKS) were analysed by liquid chromatography-electrospray ionization mass spectrometry. The antioxidan, anticancer effect on HepG2 cell and antibacterial properties of the AKS polyphenol extracts were further characterized in vitro. Polyphenol compounds (35), including nine phenolic acids, thirteen anthocyanins and thirteen flavonoids, were identified in AKS for the first time. The content of apigenin 7-O-glucoside, (cyanidin 3-(4″-acetylrutinoside), 3- (6″-acetylglucoside)-5-glucoside and salicylic acid was relatively high than the others. The AKS polyphenols strongly reduced Fe³⁺ and exhibited good scavenging activity towards 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free radicals, 1,1-diphenyl-1-picrylhydrazyl free radicals, hydroxyl radicals, superoxide anions and hydrogen peroxide. The AKS polyphenols could regulate oxidant stress in HepG2 cells by downregulating reduced glutathione, upregulating oxidative glutathione, malondialdehyde and advanced oxidation protein products, and reduced cell viability to induce apoptosis of HepG2 cells in vitro. The AKS polyphenols showed strong antibacterial activity against Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Acetobacter aceti and Bacillus cereus. Therefore, the antioxidant, inhibitory effect on HepG2 cells and antimicrobial activity of the AKS polyphenols were distinct and worthy of further consideration for medical industry applications.

Keywords: Antibacterial; Antioxidant; Apricot kernel skin; HepG2 cells; Polyphenols.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Antioxidants / chemistry
Antioxidants / pharmacology*
Apoptosis / drug effects
Bacillus / drug effects
Escherichia coli / drug effects
Hep G2 Cells
Humans
Hydrogen Peroxide / chemistry
Microbial Sensitivity Tests
Polyphenols / analysis*
Polyphenols / pharmacology*
Prunus armeniaca / chemistry*
Staphylococcus aureus / drug effects

Substances

Anti-Bacterial Agents
Antioxidants
Polyphenols
Hydrogen Peroxide