Engineered xCas9 and SpCas9-NG variants broaden PAM recognition sites to generate mutations in Arabidopsis plants

Plant Biotechnol J. 2019 Oct;17(10):1865-1867. doi: 10.1111/pbi.13148. Epub 2019 May 29.

Authors

Zengxiang Ge¹, Leiqi Zheng¹, Yuling Zhao¹, Jiahao Jiang¹, Emily J Zhang², Tianxu Liu¹, Hongya Gu^{1

3}, Li-Jia Qu^{1

3}

Affiliations

¹ State Key Laboratory for Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences at College of Life Sciences, Peking University, Beijing, China.
² Aragon High School, San Mateo, CA, USA.
³ The National Plant Gene Research Center (Beijing), Beijing, China.

No abstract available

Keywords: Arabidopsis; NG PAMs; SpCas9-NG; genome editing; xCas9.

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis*
CRISPR-Cas Systems*
Gene Editing*
Mutagenesis*
Plants, Genetically Modified