Biological and molecular characterization of a bacteriophage infecting Xanthomonas campestris pv. campestris, isolated from brassica fields

Arch Virol. 2019 Jul;164(7):1857-1862. doi: 10.1007/s00705-019-04263-4. Epub 2019 May 7.

Abstract

Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot of crucifers. Here, we report a virus that infects Xcc isolated from brassica fields in Brazil. Morphological, molecular and phylogenetic analysis indicated that the isolated virus is a new member of the genus Pbunavirus, family Myoviridae, and we propose the name "Xanthomonas virus XC 2" for this virus. The isolated virus has a narrow host range, infecting only Xcc isolates, and it did not infect unrelated bacteria. These results indicate that the isolated bacteriophage is highly specific for Xcc and may be a potential agent for biological control.

MeSH terms

  • Bacteriophages / classification*
  • Bacteriophages / genetics
  • Bacteriophages / isolation & purification*
  • Biological Control Agents
  • Brassica / microbiology*
  • Brazil
  • DNA, Viral / genetics
  • Genome, Viral / genetics
  • Host Specificity
  • Myoviridae / classification*
  • Myoviridae / genetics
  • Myoviridae / isolation & purification*
  • Plant Diseases / microbiology
  • Plant Diseases / therapy
  • Xanthomonas campestris / isolation & purification
  • Xanthomonas campestris / virology*

Substances

  • Biological Control Agents
  • DNA, Viral