Molecular evidence of hemotropic mycoplasmas in goats from Cebu, Philippines

J Vet Med Sci. 2019 Jun 21;81(6):869-873. doi: 10.1292/jvms.19-0042. Epub 2019 Apr 29.

Abstract

Hemotropic mycoplasma (hemoplasma), a neglected vector-borne pathogen in goats, causes extensive economic damage to farmers due to production losses. In this study, 107/295 (36.27%) goats sampled from 4 farms (Barili, Danao City, Dumanjug and Minglanilla) in Cebu, Philippines tested positive for PCR targeting the 16S rRNA gene of Mycoplasma. All hemoplasma-positive goats were from Barili and no clinical sign was observed. Sex (P=0.0005) and age (P=0.03) were found associated with hemoplasma infection. Mycoplasma ovis, Candidatus Mycoplasma haemobos, Candidatus Mycoplasma haemominutum and 3 Uncultured Mycoplasma sp. sequences were identified by sequencing analysis. This is the first report of molecular detection and genetic characterization of hemoplasmas in goats in the Philippines.

Keywords: Mycoplasma; Philippines; goat; hemoplasma.

MeSH terms

  • Age Factors
  • Animals
  • DNA, Bacterial
  • Female
  • Goat Diseases / epidemiology*
  • Goat Diseases / microbiology
  • Goats
  • Male
  • Mycoplasma / classification
  • Mycoplasma / genetics
  • Mycoplasma / isolation & purification*
  • Mycoplasma Infections / veterinary*
  • Philippines / epidemiology
  • Polymerase Chain Reaction / veterinary
  • RNA, Ribosomal, 16S
  • Sex Factors

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S