Fluorescence correlation spectroscopy in the nanosecond time range: rotational diffusion of bovine carbonic anhydrase B

Eur Biophys J. 1987;14(4):257-61. doi: 10.1007/BF00256359.

Abstract

A fluorescence correlation experiment for measurement of rotational diffusion in the nanosecond time scale is described. Using this method, the rotational diffusion coefficient of bovine carbonic anhydrase B labelled with tetramethylrhodamine isothiocyanate was estimated to be Dr = (1.14 +/- 0.15) X 10(7) s-1 at 22 degrees C. The experiment is based on a cw argon ion laser, a microfluorometer with local solution flow inside the sample cell, and two photon detectors. The fluorescence intensity autocorrelation function in the nanosecond time range is computed with the help of a time-to-amplitude converter and a multichannel pulse-amplitude analyser.

MeSH terms

  • Animals
  • Carbonic Anhydrases / metabolism*
  • Cattle
  • Fluorescent Dyes
  • Kinetics
  • Rhodamines
  • Spectrometry, Fluorescence
  • Time Factors

Substances

  • Fluorescent Dyes
  • Rhodamines
  • tetramethylrhodamine isothiocyanate
  • Carbonic Anhydrases