Interferon-Gamma test for the detection of Mycobacterium tuberculosis complex infection in Macaca mulatta and other non-human primates

J Med Primatol. 2019 Aug;48(4):260-263. doi: 10.1111/jmp.12420. Epub 2019 May 6.

Abstract

We have formatted an assay to detect Mycobacterium tuberculosis complex infections of non-human primates. Commercially available reagents were used to elicit a specific immune response that was measured by interferon-gamma release. Initial evaluation using blood samples from Rhesus macaques experimentally infected with M tuberculosis distinguished infected versus uninfected animals.

Keywords: cell-mediated immune response (CMI); interferon-gamma release assay (IGRA); purified protein derivative-tuberculin (PPD).

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Interferon-gamma Release Tests / methods
  • Interferon-gamma Release Tests / veterinary*
  • Macaca mulatta*
  • Monkey Diseases / diagnosis*
  • Mycobacterium tuberculosis / isolation & purification*
  • Tuberculosis / diagnosis*