Adipocytokines may delay pubertal maturation of human Sertoli cells

I V Wagner; P Yango; K Svechnikov; N D Tran; O Söder

doi:10.1071/RD18487

Adipocytokines may delay pubertal maturation of human Sertoli cells

Reprod Fertil Dev. 2019 Jul;31(8):1395-1400. doi: 10.1071/RD18487.

Authors

I V Wagner¹, P Yango², K Svechnikov³, N D Tran², O Söder³

Affiliations

¹ Karolinska Institutet, Department of Women's and Children's Health, Pediatric Endocrinology Unit, 17176 Stockholm, Sweden; and Department of Pediatrics, University Hospital Cologne, Kerpener Str. 62, 50937, Cologne, Germany; and Corresponding author.
² Center for Reproductive Sciences, University of California, San Francisco, 513 Parnassus Avenue, CA 94143, USA.
³ Karolinska Institutet, Department of Women's and Children's Health, Pediatric Endocrinology Unit, 17176 Stockholm, Sweden.

PMID: 31056083
DOI: 10.1071/RD18487

Abstract

Reproduction is an important target of obesity complications, including adverse effects on spermatogenesis and steroidogenesis. Adipocytokines are key mediators in various complications of obesity. Our aim was to study the potential of adipocytokines to affect Sertoli cell function, which is crucial for spermatogenesis, and possibly link these findings to the observed attenuation of spermatogenesis in obese males. Testicular biopsies were obtained from healthy donors. Highly purified adult human Sertoli cells (HSCs) were isolated by fluorescence-activated cell sorting. Cells were cultured and exposed to different concentrations of adipocytokines (10-1000ngmL-1 ) for 2-7 days. Expression of selected Sertoli cell genes was quantified by quantitative polymerase chain reaction. Long-term treatment (7 days) of HSCs with higher concentrations of chemerin, irisin, nicotinamide phosphoribosyltransferase (Nampt), resistin and progranulin significantly suppressed FSH receptor expression (by 79%, 83%, 64%, 71% and 26% respectively; P P invitro , may negatively affect Sertoli cell maturation and retain these cells in a more prepubertal stage. This could negatively affect testis function and add to fertility problems in obese adults.