Membrane selection and optimisation of tangential flow ultrafiltration of Cyclopia genistoides extract for benzophenone and xanthone enrichment

Neil Miller; Stephanie C Bosman; Christiaan J Malherbe; Dalene De Beer; Elizabeth Joubert

doi:10.1016/j.foodchem.2019.04.047

Membrane selection and optimisation of tangential flow ultrafiltration of Cyclopia genistoides extract for benzophenone and xanthone enrichment

Food Chem. 2019 Sep 15:292:121-128. doi: 10.1016/j.foodchem.2019.04.047. Epub 2019 Apr 12.

Authors

Neil Miller¹, Stephanie C Bosman², Christiaan J Malherbe³, Dalene De Beer⁴, Elizabeth Joubert⁵

Affiliations

¹ Plant Bioactives Group, Post-Harvest and Agro-Processing Technologies, Agricultural Research Council (ARC) Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa; Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch 7602, South Africa. Electronic address: MillerN@arc.agric.za.
² Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch 7602, South Africa.
³ Plant Bioactives Group, Post-Harvest and Agro-Processing Technologies, Agricultural Research Council (ARC) Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa. Electronic address: MalherbeCh@arc.agric.za.
⁴ Plant Bioactives Group, Post-Harvest and Agro-Processing Technologies, Agricultural Research Council (ARC) Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa; Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch 7602, South Africa. Electronic address: DBeerD@arc.agric.za.
⁵ Plant Bioactives Group, Post-Harvest and Agro-Processing Technologies, Agricultural Research Council (ARC) Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa; Department of Food Science, Stellenbosch University, Private Bag X1, Matieland, Stellenbosch 7602, South Africa. Electronic address: JoubertL@arc.agric.za.

PMID: 31054655
DOI: 10.1016/j.foodchem.2019.04.047

Abstract

Ultrafiltration of Cyclopia genistoides extract was optimised to increase its benzophenone and xanthone content as quantified using HPLC-DAD. Regenerated cellulose (RC) and polyethersulphone membranes with molecular weight cut-offs of 10 and 30 kDa were evaluated in terms of compound enrichment, permeate flux and permeate yield, using dead-end ultrafiltration. Compound enrichment was subsequently optimised using the 10 kDa RC membrane and tangential flow ultrafiltration (TFU). The effect of extract composition on compound enrichment, due to natural variation in the source material, was assessed using extracts from different batches of plant material (n = 11). Transmembrane pressure and feed flow rate affected (p < 0.05) process efficiency (mean permeate flux, compound enrichment and membrane fouling). TFU achieved ≥20% enrichment of the target compounds, proving its suitability for preparation of a nutraceutical extract of C. genistoides.

Keywords: 3-β-d-Glucopyranosyliriflophenone (PubChem CID: 53396784); 3-β-d-Glucopyranosylmaclurin (PubChem CID: 101876647); Benzophenones; Enrichment; Hesperidin (PubChem CID: 10621); Honeybush; Isomangiferin (PubChem CID: 5318597); Mangiferin (PubChem CID: 5281647); Ultrafiltration; Vicenin-2 (PubChem CID: 442664); Xanthones.

MeSH terms

Benzophenones / analysis*
Benzophenones / isolation & purification
Chromatography, High Pressure Liquid
Fabaceae / metabolism*
Membranes, Artificial
Plant Extracts / chemistry*
Ultrafiltration / methods*
Xanthones / analysis*
Xanthones / isolation & purification

Substances

Benzophenones
Membranes, Artificial
Plant Extracts
Xanthones
benzophenone
xanthone