Charge-Dependent Regulation in DNA Adsorption on 2D Clay Minerals

Hongyi Xie; Zhengqing Wan; Song Liu; Yi Zhang; Jieqiong Tan; Huaming Yang

doi:10.1038/s41598-019-41093-5

Charge-Dependent Regulation in DNA Adsorption on 2D Clay Minerals

Sci Rep. 2019 May 2;9(1):6808. doi: 10.1038/s41598-019-41093-5.

Authors

Hongyi Xie^{1

2}, Zhengqing Wan³, Song Liu⁴, Yi Zhang^{5

6}, Jieqiong Tan⁷, Huaming Yang^{8

9}

Affiliations

¹ Department of Inorganic Materials, School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.
² Hunan Key Lab of Mineral Materials and Application, Central South University, Changsha, 410083, China.
³ The Center for Medical Genetics, School of Life Science, Central South University, Changsha, 410078, China.
⁴ Institute of Chemical Biology and Nanomedicine, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
⁵ Department of Inorganic Materials, School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China. yee_z10@csu.edu.cn.
⁶ Hunan Key Lab of Mineral Materials and Application, Central South University, Changsha, 410083, China. yee_z10@csu.edu.cn.
⁷ The Center for Medical Genetics, School of Life Science, Central South University, Changsha, 410078, China. tanjieqiong@sklmg.edu.cn.
⁸ Department of Inorganic Materials, School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China. hmyang@csu.edu.cn.
⁹ Hunan Key Lab of Mineral Materials and Application, Central South University, Changsha, 410083, China. hmyang@csu.edu.cn.

Abstract

DNA purification is essential for the detection of human clinical specimens. A non-destructive, controllable, and low reagent consuming DNA extraction method is described. Negatively charged DNA is absorbed onto a negatively charged montmorillonite to achieve non-destructive DNA extraction based on cation bridge construction and electric double layer formation. Different valence cation modified montmorillonite forms were used to validate the charge-dependent nature of DNA adsorption on montmorillonite. Electric double layer thickness thinning/thickening with the high/lower valence cations exists, and the minerals tended to be sedimentation-stable due to the Van der Waals attraction/electrostatic repulsion. Li-modified montmorillonite with the lowest charge states showed the best DNA adsorption efficiency of 8-10 ng/μg. Charge-dependent regulating research provides a new perspective for controllable DNA extraction and a deep analysis of interface engineering mechanisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Algorithms
Cations
Clay / chemistry*
DNA / chemistry*
DNA / isolation & purification*
Minerals / chemistry*
Models, Theoretical
Static Electricity

Substances

Cations
Minerals
DNA
Clay