Soil solid-phase organic matter-mediated microbial reduction of iron minerals increases with land use change sequence from fallow to paddy fields

Abstract

The microbial reduction of Fe(III) minerals (MRF) is an important process in paddy soil because it can affect the biogeochemical cycles of many major and trace elements. Natural organic matter (NOM) that mainly exists in the form of solid phase in soil can mediate MRF through electron shuttling functionality. However, whether a link exists between solid-phase NOM-mediated MRF in soil and the age of paddy field since the reclamation on fallow is unclear. Here, we use microbial reduction method to assess the solid-phase NOM-mediated MRF of paddy soils with different reclamation ages. The results show that solid-phase NOM-mediated MRF exhibits a positive response to land use change sequence from fallow to paddy field, indicating that the long-term natural development of paddy field favors the electron shuttling of NOM between cells and Fe(III) minerals. This increase in the electron shuttling of NOM is not due to the increase in the redox functional groups of NOM, but may be attributed to the formation of NOM-mineral complex through the synergistic increases in NOM content and transformation of soil texture from clay loam to loam. The decrease in the redox potential of Fe(III) minerals in soil caused by decreased pH and the increase in Fe content in the organic matter-complexed form may also partly facilitate electron transfer from NOM to Fe(III) minerals. Our work is useful in predicting the role of soil solid-phase NOM in mediating MRF in the context of long-term reclamation of paddy field and provides guidance for the environmental management of paddy fields.

Keywords: In situ soil organic matter; Land use change sequence; Microbial reduction of Fe(III) minerals; Paddy soil.