Purification of the Recombinant Adenovirus by Cesium Chloride Gradient Centrifugation

Cold Spring Harb Protoc. 2019 May 1;2019(5). doi: 10.1101/pdb.prot095547.

Abstract

Cesium chloride gradient centrifugation is the most widely used method for purification of recombinant adenovirus. This protocol describes the entire process, from the preparation and clarification of crude viral lysate to the formulation and storage of purified virus.

MeSH terms

  • Adenoviridae / genetics*
  • Adenoviridae / isolation & purification*
  • Centrifugation, Density Gradient / methods*
  • Cesium / chemistry*
  • Chlorides / chemistry*
  • Genetic Vectors / metabolism
  • Recombination, Genetic* / genetics

Substances

  • Chlorides
  • Cesium
  • cesium chloride