Release of the Cloned Recombinant Adenovirus Genome for Rescue and Expansion

Cold Spring Harb Protoc. 2019 May 1;2019(5). doi: 10.1101/pdb.prot095539.

Abstract

After generating the recombinant adenovirus plasmid by direct cloning, the viral genome must be released from the plasmid for virus rescue and expansion in corresponding packaging cell lines. This protocol describes how to process and rescue a recombinant adenovirus and grow the rescued virus to large scale.

MeSH terms

  • Adenoviridae / genetics*
  • Adenoviridae / isolation & purification
  • Cloning, Molecular / methods*
  • DNA, Recombinant / genetics*
  • Genetic Vectors / metabolism
  • Genome, Viral*
  • HEK293 Cells
  • Humans
  • Plasmids / genetics
  • Transfection

Substances

  • DNA, Recombinant