Efficient Phytase Secretion and Phytate Degradation by Recombinant Bifidobacterium longum JCM 1217

Zhongke Sun; Zonghao Yue; Xingdong Yang; Xinqi Hao; Maoping Song; Lili Li; Can Chen; Cuiwei Chu; Chengwei Li

doi:10.3389/fmicb.2019.00796

Efficient Phytase Secretion and Phytate Degradation by Recombinant Bifidobacterium longum JCM 1217

Front Microbiol. 2019 Apr 16:10:796. doi: 10.3389/fmicb.2019.00796. eCollection 2019.

Authors

Zhongke Sun^{1

2}, Zonghao Yue¹, Xingdong Yang¹, Xinqi Hao², Maoping Song², Lili Li^{1

3}, Can Chen¹, Cuiwei Chu¹, Chengwei Li^{1

3}

Affiliations

¹ College of Life Sciences and Agronomy, Zhoukou Normal University, Zhoukou, China.
² College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
³ Key Laboratory of Plant Molecular Breeding and Bioreactor, Zhoukou, China.

Abstract

Genetic engineering of probiotics, like bifidobacteria, may improve their microbial cell factory economy. This work designed a novel shuttle plasmid pBPES, which bears exogenous appA and is stable within Bifidobacterium longum JCM 1217. Cloning of three predicted promoters into pBPES proved that all of them drive appA expression in B. longum JCM 1217. Transformation of plasmids pBPES-tu and pBPES-groEL into B. longum JCM1217 resulted in much more phytase secretion suggests P _tu and P _groEL are strong promoters. Further in vitro and in vivo experiments suggested B. longum JCM 1217/pBPES-tu degrades phytate efficiently. In conclusion, the study screened two stronger promoters and constructed a recombinant live probiotic strain for effectively phytase secretion and phytate degradation in gut. The strategy used in the study provided a novel technique for improving the bioaccessibility of phytate and decreasing phosphorus excretion.

Keywords: Bifidobacterium longum JCM 1217; phosphorus; phytase; phytate; promoter.