In this paper we report the development of a highly sensitive amperometric glycerol biosensor based on alcohol dehydrogenase from Pseudomonas putida immobilized on graphite electrode modified with carbon nanotubes and a redox mediator tetrathiafulvalene. The designed biosensor demonstrates very high sensitivity towards glycerol (29.2 ± 0.9 µA mM-1 cm-2), low limit of detection (18 µM), linear range from 0.05 to 1.0 mM, high selectivity and satisfactory stability. Biosensor has been successfully used for the determination of glycerol concentration in buffer solutions as well as in the human urine samples. Received results shows a satisfactory agreement with the control measurements carried out using colorimetric commercially available glycerol determination assay kit, thus developed biosensor can be successfully applied for measurements of glycerol concentration in human urine and may be a fast, attractive and non-invasive tool for the determination of glycerol.
Keywords: Alcohol dehydrogenase; Amperometric biosensor; Carbon nanotubes; Glycerol; Tetrathiafulvalene.
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