Real time monitoring of glucose in whole blood by smartphone

Miguel M Erenas; Belén Carrillo-Aguilera; Kevin Cantrell; Sara Gonzalez-Chocano; Isabel Maria Perez de Vargas-Sansalvador; Ignacio de Orbe-Payá; Luis Fermin Capitan-Vallvey

doi:10.1016/j.bios.2019.04.024

Real time monitoring of glucose in whole blood by smartphone

Biosens Bioelectron. 2019 Jul 1:136:47-52. doi: 10.1016/j.bios.2019.04.024. Epub 2019 Apr 22.

Authors

Miguel M Erenas¹, Belén Carrillo-Aguilera², Kevin Cantrell³, Sara Gonzalez-Chocano², Isabel Maria Perez de Vargas-Sansalvador⁴, Ignacio de Orbe-Payá⁴, Luis Fermin Capitan-Vallvey⁴

Affiliations

¹ ECsens., Department of Analytical Chemistry, Faculty of Science, Campus Fuentenueva, University of Granada, 18071, Spain; Unit of Excellence in Chemistry applied to Biomedicine and the Environment of the University of Granada, Faculty of Sciences, Campus Fuentenueva, University of Granada, 18071, Spain. Electronic address: erenas@ugr.es.
² ECsens., Department of Analytical Chemistry, Faculty of Science, Campus Fuentenueva, University of Granada, 18071, Spain.
³ Department of Chemistry, The University of Portland, 5000 N Willamette Blvd, Portland, OR, 97203, USA.
⁴ ECsens., Department of Analytical Chemistry, Faculty of Science, Campus Fuentenueva, University of Granada, 18071, Spain; Unit of Excellence in Chemistry applied to Biomedicine and the Environment of the University of Granada, Faculty of Sciences, Campus Fuentenueva, University of Granada, 18071, Spain.

PMID: 31035026
DOI: 10.1016/j.bios.2019.04.024

Abstract

A combined thread-paper microfluidic device (μTPAD) is presented for the determination of glucose in blood. The device is designed to include all the analytical operations needed: red blood cell separation, conditioning, enzymatic recognition, and colorimetric transduction. The signal is captured with a smartphone or tablet working in video mode and processed by custom Android-based software in real-time. The automatic detection of the region of interest on the thread allows for the use of either initial rate or equilibrium signal as analytical parameters. The time needed for analysis is 12 s using initial rate, and 100 s using the equilibrium measurement with a LOD of 48 μM and 12 μM, respectively, and a precision around 7%. The μTPAD allows a rapid determination of glucose in real samples using only 3 μL of whole blood.

Keywords: Glucose determination; Reaction rate; Smartphone; Thread-paper microfluidic device; Whole blood.

MeSH terms

Blood Glucose / analysis*
Colorimetry / instrumentation
Humans
Lab-On-A-Chip Devices*
Smartphone*
Software

Substances

Blood Glucose