A novel Ca2+-binding protein influences photosynthetic electron transport in Anabaena sp. PCC 7120

Abstract

Ca²⁺ is a potent signalling molecule that regulates many cellular processes. In cyanobacteria, Ca²⁺ has been linked to cell growth, stress response and photosynthesis, and to the development of specialist heterocyst cells in certain nitrogen-fixing species. Despite this, the pathways of Ca²⁺ signal transduction in cyanobacteria are poorly understood, and very few protein components are known. The current study describes a previously unreported Ca²⁺-binding protein which was called the Ca²⁺ Sensor EF-hand (CSE), which is conserved in filamentous, nitrogen-fixing cyanobacteria. CSE is shown to bind Ca²⁺, which induces a conformational change in the protein structure. Poor growth of a strain of Anabaena sp. PCC 7120 overexpressing CSE was attributed to diminished photosynthetic performance. Transcriptomics, biophysics and proteomics analyses revealed modifications in the light-harvesting phycobilisome and photosynthetic reaction centre protein complexes.

Keywords: Anabaena; Calcium; Cyanobacteria; EF-hand; Photosynthesis; Phycobilisomes.