Zinc fortification as a tool for improving sprout hygienic and nutritional quality: a factorial design approach

J Sci Food Agric. 2019 Aug 30;99(11):5187-5194. doi: 10.1002/jsfa.9765. Epub 2019 May 24.

Abstract

Background: Sprouting is known to improve cereal and pulse nutritional properties. However, several outbreaks of illness have been reported after raw sprout consumption. This research aimed to improve wheat sprout hygienic properties through the use of zinc diacetate. Sprouting conditions (sprouting temperature, soaking time and zinc diacetate solution concentration) were optimized to decrease total plate count, coliforms, and molds and yeasts using a factorial design approach and a desirability function.

Results: Based on the responses, the effects of variables were calculated and the interactions between them were determined. Optimal conditions were defined as follows: sprouting temperature 18 °C, soaking time 0.66 h and zinc diacetate concentration 400 mg L-1 . These conditions led to the elimination of coliforms and a decrease in total flora count by 2 log. Interestingly, zinc sprouting increased the zinc content of sprouts and improved their nutritional properties.

Conclusion: Results showed that the use of zinc solution is a useful tool to improve sprout hygienic and nutritional properties. © 2019 Society of Chemical Industry.

Keywords: durum wheat; factorial design; hygienic properties; sprouting; zinc.

Publication types

  • Evaluation Study

MeSH terms

  • Bacteria / growth & development
  • Culture Media / chemistry
  • Culture Media / metabolism
  • Food Contamination / prevention & control*
  • Food Handling / instrumentation
  • Food Handling / methods*
  • Fungi / growth & development
  • Hygiene
  • Nutritive Value
  • Seeds / chemistry
  • Seeds / growth & development*
  • Seeds / microbiology
  • Triticum / chemistry
  • Triticum / growth & development*
  • Triticum / microbiology
  • Zinc / analysis
  • Zinc / pharmacology*

Substances

  • Culture Media
  • Zinc