Effects of seaweed fertilizer on enzyme activities, metabolic characteristics, and bacterial communities during maize straw composting

Abstract

The objective of this study was to investigate microbial responses when seaweed fertilizer was added to maize straw compost. The metabolic characteristics and bacterial communities were assessed using 16S rRNA sequencing and Biolog analysis. Results showed that the urease, dehydrogenase, and metabolic activities improved on day 21 after the addition of seaweed fertilizer. Firmicutes, Proteobacteria, and Actinobacteria were the dominant phyla in the compost. The relative abundances of Proteobacteria and Actinobacteria were increased by addition of seaweed fertilizer during the early composting stage. Temperature, NH₄⁺-N, NO₃^--N concentrations, and carbon source metabolism had close relationships with bacterial community variation during composting. A network analysis revealed that NO₃^--N content had a positive association with Aeromicrobium, and seaweed fertilizer application improved the growth of keystone species related to N cycling. These results suggested that seaweed fertilizer influenced bacterial community succession through its effects on N concentrations during the composting process.

Keywords: Enzyme activity; High-throughput sequencing; Metabolic capacity; Network analysis; Seaweed fertilizer.