Coumarin-based Hg2+ fluorescent probe: Fluorescence turn-on detection for Hg2+ bioimaging in living cells and zebrafish

Chong-Guang Chen; Natarajan Vijay; Natesan Thirumalaivasan; Sivan Velmathi; Shu-Pao Wu

doi:10.1016/j.saa.2019.04.048

Coumarin-based Hg²⁺ fluorescent probe: Fluorescence turn-on detection for Hg²⁺ bioimaging in living cells and zebrafish

Spectrochim Acta A Mol Biomol Spectrosc. 2019 Aug 5:219:135-140. doi: 10.1016/j.saa.2019.04.048. Epub 2019 Apr 19.

Authors

Chong-Guang Chen¹, Natarajan Vijay², Natesan Thirumalaivasan¹, Sivan Velmathi³, Shu-Pao Wu⁴

Affiliations

¹ Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan.
² Organic and Polymer Synthesis Laboratory, Department of Chemistry, National Institute of Technology, Tiruchirappalli 620 015, India.
³ Organic and Polymer Synthesis Laboratory, Department of Chemistry, National Institute of Technology, Tiruchirappalli 620 015, India. Electronic address: velmathis@nitt.edu.
⁴ Department of Applied Chemistry, National Chiao Tung University, Hsinchu 300, Taiwan. Electronic address: spwu@mail.nctu.edu.tw.

PMID: 31030041
DOI: 10.1016/j.saa.2019.04.048

Abstract

The need in developing fluorescent probes for trace metal ion detection in biological samples has been an important issue. Herein, a reaction-based fluorescent probe PIC containing a perimidine moiety was designed and synthesized for Hg²⁺ detection. The probe can selectively distinguish Hg²⁺ with 42-fold fluorescent enhancement from the other metal ions at physiological pH. This probe can detect Hg²⁺ with the detection limit of 1.08 μM. The sensor PIC can be applied to real-time detection of Hg²⁺ in cells with blue emission.

Keywords: Bioimaging; Coumarin; Hg(II); Sensor; Zebrafish.

MeSH terms

Animals
Cations, Divalent / analysis
Coumarins / chemistry*
Fluorescent Dyes / chemistry*
HeLa Cells
Humans
Mercury / analysis*
Optical Imaging / methods
Spectrometry, Fluorescence / methods
Zebrafish / embryology

Substances

Cations, Divalent
Coumarins
Fluorescent Dyes
Mercury