Optimization of the reduction of 74As(V) to 74As(III) and of the labelling of dithiol dihydrolipoic acid

Zita Oláh; Andreas T J Vogg; Tibor Kremmer; Zoltán Szűcs; Zoltán Varga; Rita Dóczi

doi:10.1016/j.apradiso.2019.04.004

Optimization of the reduction of ⁷⁴As(V) to ⁷⁴As(III) and of the labelling of dithiol dihydrolipoic acid

Appl Radiat Isot. 2019 Jul:149:75-82. doi: 10.1016/j.apradiso.2019.04.004. Epub 2019 Apr 15.

Authors

Zita Oláh¹, Andreas T J Vogg², Tibor Kremmer³, Zoltán Szűcs⁴, Zoltán Varga⁵, Rita Dóczi⁶

Affiliations

¹ National Food Chain Safety Office, Radioanalytical Reference Laboratory, Fogoly street 13-15, H-1182, Budapest, Hungary.
² Department of Nuclear Medicine, RWTH University Hospital Aachen, Pauwelsstr. 30, D-52074, Aachen, Germany.
³ Institute of Chemistry, Faculty of Science, Eötvös Loránd University, Pázmány Péter sétány 1/A, H-1117, Budapest, Hungary.
⁴ Institute for Nuclear Research of the Hungarian Academy of Sciences, 4+Bem tér 18/c, H-4026, Debrecen, Hungary. Electronic address: zszucs@atomki.hu.
⁵ Institute of Materials and Environmental Chemistry, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Magyar tudósok körútja 2, H-1117, Budapest, Hungary.
⁶ Budapest University of Technology and Economics, Institute of Nuclear Techniques, 7-9 Műegyetem rkp, H-1111, Budapest, Hungary.

PMID: 31029937
DOI: 10.1016/j.apradiso.2019.04.004

Abstract

The radiochemical separation of n.c.a. arsenic on its own or for radio-labelling purposes usually involves the issue of reducing arsenic(V). Numerous approaches for reducing pentavalent arsenic have been examined. A novel HPLC method has also been presented for accessing the efficiency of the reduction in terms of *As(III)/*As(V). Labelling with trivalent radioarsenic seems to be a promising research field to access new radiopharmaceuticals, for example, using arsenic as a surrogate for phosphorus. Moreover, as a model system, the labelling reaction of *As(III) with dihydrolipoic acid has been systematically optimized.