New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds

Wei Xi; Zongxiang Tang; Shuyao Tang; Zujun Yang; Jie Luo; Shulan Fu

doi:10.3390/ijms20082031

New ND-FISH-Positive Oligo Probes for Identifying Thinopyrum Chromosomes in Wheat Backgrounds

Int J Mol Sci. 2019 Apr 25;20(8):2031. doi: 10.3390/ijms20082031.

Authors

Wei Xi^{1

2}, Zongxiang Tang^{3

4}, Shuyao Tang^{5

6}, Zujun Yang⁷, Jie Luo^{8

9}, Shulan Fu^{10

11}

Affiliations

¹ Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. xiwei923700915@163.com.
² College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. xiwei923700915@163.com.
³ Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. zxtang@sicau.edu.cn.
⁴ College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. zxtang@sicau.edu.cn.
⁵ Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. tangshuyao705708@sina.com.
⁶ College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. tangshuyao705708@sina.com.
⁷ Center for Informational Biology, University of Electronic Science and Technology of China, Chengdu 610054, Sichuan, China. yangzujun@uestc.edu.cn.
⁸ Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. luojie2010@sina.com.
⁹ College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. luojie2010@sina.com.
¹⁰ Provincial Key Laboratory of Plant Breeding and Genetics, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. fushulan@sicau.edu.cn.
¹¹ College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu 611130, Sichuan, China. fushulan@sicau.edu.cn.

Abstract

Thinopyrum has been widely used to improve wheat (Triticum aestivum L.) cultivars. Non-denaturing fluorescence in situ hybridization (ND-FISH) technology using oligonucleotides (oligo) as probes provides a convenient and efficient way to identify alien chromosomes in wheat backgrounds. However, suitable ND-FISH-positive oligo probes for distinguishing Thinopyrum chromosomes from wheat are lacking. Two oligo probes, Oligo-B11 and Oligo-pThp3.93, were designed according to the published Thinopyrum ponticum (Th. ponticum)-specific repetitive sequences. Both Oligo-B11 and Oligo-pThp3.93 can be used for ND-FISH analysis and can replace conventional GISH and FISH to discriminate some chromosomes of Th. elongatum, Th. intermedium, and Th. ponticum in wheat backgrounds. The two oligo probes provide a convenient way for the utilization of Thinopyrum germplasms in future wheat breeding programs.

Keywords: ND-FISH; Thinopyrum; chromosome; oligo probe; wheat.

MeSH terms

Chromosomes, Plant / genetics*
DNA Probes / metabolism*
In Situ Hybridization, Fluorescence / methods*
Metaphase
Nucleic Acid Denaturation*
Poaceae / genetics*
Triticum / genetics*

Substances

DNA Probes

Abstract

MeSH terms

Substances

Grants and funding